Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases

In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated made. DNA isolations different purified colonies carried out with the mericon bacterial kit. Real-Time PCR procedure was performed by using universal primers. Molecular identifications blasting nucleotides obtained sequence analysis amplicons. Spectrophotometric measurements at 412 nm wavelengths for AChE activity BChE liver brain tissues samples. The differences disease factors among themselves according to control group examined. As a result isolation Bacillus subtilis, Lactococcus garvieae Staphylococcus epidermidis 5 farms performed. Over 98% similarity observed as sequencing isolates. it that three bacteria suppressed enzyme activities in both trout.